Journal: Integrative Medicine: A Clinician's Journal

Article Title: Probiotics and Disease: A Comprehensive Summary—Part 5, Respiratory Conditions of the Ears, Nose, and Throat

doi:

Figure Lengend Snippet: Ears, Nose, and Throat; Oral Health; and Respiratory Tract

Article Snippet: Pneumonia , Advanced Multi-Billion Dophilus (Solgar; L acidophilus, LA-5, B lactis, BB-12, L paracasei, L casei 431, L rhamnosus GG, LGG) , L casei, L rhamnosus EO1-AO2-S06, L casei.

Techniques: Cell Culture, Activity Assay, Isolation, Bacteria, Probiotics, Immunopeptidomics, In Vitro

Journal: Integrative Medicine: A Clinician's Journal

Article Title: Probiotics and Disease: A Comprehensive Summary—Part 5, Respiratory Conditions of the Ears, Nose, and Throat

doi:

Figure Lengend Snippet: Summary of Nutritional Supplements by Health Concern

Article Snippet: Pneumonia , Advanced Multi-Billion Dophilus (Solgar; L acidophilus, LA-5, B lactis, BB-12, L paracasei, L casei 431, L rhamnosus GG, LGG) , L casei, L rhamnosus EO1-AO2-S06, L casei.

Techniques: Probiotics, Starch

Journal: Scientific Reports

Article Title: In vivo detection of small tumour lesions by multi-pinhole SPECT applying a 99m Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor

doi: 10.1038/srep21834

Figure Lengend Snippet: Western blot analysis of EGFR expression levels of cell lysates of human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 as well as of the human epidermoid carcinoma cell line A431 are shown. A high EGFR expression is found in MDA-MB-468 and A431 cells in comparison to MDA-MB-231 cells, which only express low amounts of EGFR. Actin was used as loading control.

Article Snippet: Human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 and human epidermoid carcinoma cell line A431 (DSMZ, Braunschweig, Germany) were cultured in high glucose (4.5 g/l) DMEM with Glutamax (LifeTechnologies, Darmstadt, Germany) supplemented with 10% fetal calf serum (PAA, Cöble, Germany).

Techniques: Western Blot, Expressing, Comparison, Control

Journal: Scientific Reports

Article Title: In vivo detection of small tumour lesions by multi-pinhole SPECT applying a 99m Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor

doi: 10.1038/srep21834

Figure Lengend Snippet: Binding capacities of anti-EGFR nanobody D10 (blue) and radiolabelled 99m Tc-D10 (red) on ( a ) mammary carcinoma cells MDA-MB-231 and ( b ) MDA-MB-468 as well as ( c ) epidermoid carcinoma cells A431 were investigated by flow cytometry and compared to the irrelevant control nanobody F5 (light blue) and to the radiolabelled 99m Tc-F5 (light red). Results demonstrate no alteration of the binding properties to EGFR caused by the radiolabelling procedure. All nanobodies were revealed by consecutive incubation with an anti-myc antibody and goat-anti-mouse antibody coupled to R-Phycoerythrin (PE). Mean Fluorescence Intensity (MFI) of control nanobody F5 matched the MFI values of background and negative control (i.e. incubation with anti-myc antibody and goat-anti-mouse-PE only), ruling out any unspecific binding to the tumour cells. 5 × 10 5 cells per sample were recorded and MFIs were displayed as histograms.

Article Snippet: Human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 and human epidermoid carcinoma cell line A431 (DSMZ, Braunschweig, Germany) were cultured in high glucose (4.5 g/l) DMEM with Glutamax (LifeTechnologies, Darmstadt, Germany) supplemented with 10% fetal calf serum (PAA, Cöble, Germany).

Techniques: Binding Assay, Flow Cytometry, Control, Incubation, Fluorescence, Negative Control

Journal: Scientific Reports

Article Title: In vivo detection of small tumour lesions by multi-pinhole SPECT applying a 99m Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor

doi: 10.1038/srep21834

Figure Lengend Snippet: In vivo and ex vivo determined tumour and tissue uptake of anti-EGFR nanobody 99m Tc-D10 and 99m Tc-Cetuximab.

Article Snippet: Human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 and human epidermoid carcinoma cell line A431 (DSMZ, Braunschweig, Germany) were cultured in high glucose (4.5 g/l) DMEM with Glutamax (LifeTechnologies, Darmstadt, Germany) supplemented with 10% fetal calf serum (PAA, Cöble, Germany).

Techniques: In Vivo, Ex Vivo

Journal: Scientific Reports

Article Title: In vivo detection of small tumour lesions by multi-pinhole SPECT applying a 99m Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor

doi: 10.1038/srep21834

Figure Lengend Snippet: A431 tumour bearing mice received either 17 pmol (2.6–5.1 MBq) radiolabelled anti-EGFR nanobody 99m Tc-D10 (N = 6) or 9 pmol (9.4–15.6 MBq) 99m Tc-Cetuximab (N = 5) intravenously. Ex vivo biodistribution ( a ) 100 min post injection of anti-EGFR nanobody 99m Tc-D10 and ( b ) 25 h post injection of 99m Tc-Cetuximab are shown. ( c ) Ex vivo tumour to blood ratios and tumour to tissue (muscle) ratios after nanobody 99m Tc-D10 application (100 min post injection) compared to 99m Tc-Cetuximab (25 h post injection) are presented. ( d ) In vivo SPECT scans were performed 45 min post nanobody 99m Tc-D10 and irrelevant nanobody 99m Tc-F5 injection and 24 h post 99m Tc-Cetuximab injection. The area of the tumour was segmented and compared to an equally sized contra lateral region as a measure for tissue uptake. Ratios were calculated as ratio of tumour uptake and tissue uptake. *P < 0.05, unpaired two-tailed t-Test, for in vivo tumour to tissue ratio: nanobody 99m Tc-D10 vs. irrelevant nanobody 99m Tc-F5 and nanobody 99m Tc-D10 vs. 99m Tc-Cetuximab. ***P < 0.001, unpaired two-tailed t-Test, for ex vivo tumour to blood ratio: nanobody 99m Tc-D10 vs. 99m Tc-Cetuximab.

Article Snippet: Human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 and human epidermoid carcinoma cell line A431 (DSMZ, Braunschweig, Germany) were cultured in high glucose (4.5 g/l) DMEM with Glutamax (LifeTechnologies, Darmstadt, Germany) supplemented with 10% fetal calf serum (PAA, Cöble, Germany).

Techniques: Ex Vivo, Injection, In Vivo, Single Photon Emission Computed Tomography, Two Tailed Test

Journal: Scientific Reports

Article Title: In vivo detection of small tumour lesions by multi-pinhole SPECT applying a 99m Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor

doi: 10.1038/srep21834

Figure Lengend Snippet: A431 tumour bearing mice received 17 pmol (3.3 MBq; tumour volume 22 mm 3 ) radiolabelled anti-EGFR nanobody 99m Tc-D10 or 9 pmol (10.5 MBq; tumour volume 16 mm 3 ) 99m Tc-Cetuximab intravenously. SPECT imaging was performed 45 min post 99m Tc-D10 and 24 h post 99m Tc-Cetuximab administration. Contrast-enhanced CT and SPECT scans were performed on different modalities and images were aligned by hand according to 99m Tc-pertechnetate landmarks (<30 kBq). Tumours are indicated by white arrows. Note, that a high tumour accumulation with a low background was achieved with anti-EGFR nanobody 99m Tc-D10.

Article Snippet: Human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 and human epidermoid carcinoma cell line A431 (DSMZ, Braunschweig, Germany) were cultured in high glucose (4.5 g/l) DMEM with Glutamax (LifeTechnologies, Darmstadt, Germany) supplemented with 10% fetal calf serum (PAA, Cöble, Germany).

Techniques: Single Photon Emission Computed Tomography, Imaging

Journal: Scientific Reports

Article Title: In vivo detection of small tumour lesions by multi-pinhole SPECT applying a 99m Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor

doi: 10.1038/srep21834

Figure Lengend Snippet: Anti-EGFR nanobody AF488-D10 (14 pmol) was injected in the tail vein of a nude mouse bearing a A431 tumour. ( a ) depicts an overview at 4x magnification of a slice in the middle of the tumour that shows the nanobody AF488-D10 binding on single cells and ( b ) shows a slice of half of the spleen in an upright position in 175 μm depth at 4x magnification. Due to the dehydration and clearing procedure the volume of the spleen was decreased but subcellular structures remained unmodified. Only background fluorescence and no distinct structures can be observed within the spleen.

Article Snippet: Human mammary carcinoma cell lines MDA-MB-231 and MDA-MB-468 and human epidermoid carcinoma cell line A431 (DSMZ, Braunschweig, Germany) were cultured in high glucose (4.5 g/l) DMEM with Glutamax (LifeTechnologies, Darmstadt, Germany) supplemented with 10% fetal calf serum (PAA, Cöble, Germany).

Techniques: Injection, Binding Assay, Fluorescence